High Throughput Assay Screening

Designing a Screening Campaign: Goals, Approach and Plan

“What are your goals and how do you define success?” We’ve successfully optimized and performed many types of assays. We’ll work with you to understand your goals to customize a project that meets your needs. By combining our partners’ deep knowledge of their field with the broad screening experiences of the Center, we will develop an overall strategy for success.

Assay Development

Working with you, we will develop a robust and practical assay suitable for screening. Multiple assay conditions and assay types can be quickly surveyed using 384-well plates and our specialized equipment. We use statistical measures of assay robustness, namely Signal to Background Ratio. Z’, and Coefficient of Variations for control populations, to drive assay development and assess screen readiness.

Primary Screening

Primary screening typically generates single point data using one concentration for each compound or siRNA. We normalize screening data relative to the control populations on each plate, and provide plate-based summary statistics for an evaluation of screen performance. Hit selection strategies to fit your project goals are implemented. Investigators are responsible for procuring reagents to perform their screen whereas the Center is responsible for standard laboratory consumables (pipet tips, assay plates). The price for running and analyzing a primary screen typically reflects a combination of a fee per assay plate plus an hourly fee when Center staff are needed.  For Yale faculty, these fees are largely subsidized by the Center.

For a full description of our screening collections visit our Small Molecule Collections or siRNA Library page.

Hit Picking and Dose-Response

Hit picking is performed after a primary screen to retest active compounds and to rule out compounds which exhibit assay interference or toxicity, or to validate single siRNA duplexes for their effects. Dose Response studies, using serial dilutions of each compound, can be performed within a plate, or across a series of plates in cases where many compounds are being tested. Hit selection and expansion strategies to fit your project goals are implemented. The price for setting up, running and analyzing the results is a combination of a fee per assay plate and a Center use hourly fee, which are subsidized by the Center.