sgRNA Libraries

The Horizon Discovery synthetic, single guide RNA (sgRNA) genome library targets 19,195 human genes. The sgRNAs are arrayed in sixty-one 384-well plates. Three sgRNAs targeting different regions of a single gene are combined into a single well for robust, reliable gene knockout.

Unlike pooled genome-wide libraries, this library can be screened in microscopy-based or platereader assays without the need for next-generation sequencing for deconvolution. Amenable assay types to assess the effect of loss of a single gene on cell function are numerous, including high content imaging, cell proliferation, fluorescence intensity, HTRF, BRET, reporter gene, and luminescence.

The library is made up of three subsets:

Please contact Yulia Surovtseva ( for the current per assay plate fee structure applicable to your project.