The Horizon Discovery synthetic, single guide RNA (sgRNA) genome library targets 19,195 human genes. The sgRNAs are arrayed in sixty-one 384-well plates. Three sgRNAs targeting different regions of a single gene are combined into a single well for robust, reliable gene knockout.
Unlike pooled genome-wide libraries, this library can be screened in microscopy-based or platereader assays without the need for next-generation sequencing for deconvolution. Amenable assay types to assess the effect of loss of a single gene on cell function are numerous, including high content imaging, cell proliferation, fluorescence intensity, HTRF, BRET, reporter gene, and luminescence.
The library is made up of three subsets:
- Drug Targets (12 plates - 3,683 genes) Download Drug Targets list
- Druggable Genome (15 plates – 4,643 genes) Download Druggable Genome list
- Human Genome (34 plates – 10,869 genes, estimated availability for screening: Q1 2022) Download Human Genome list
Please contact Yulia Surovtseva (email@example.com) for the current per assay plate fee structure applicable to your project.